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Published on: July 17, 2021
Genomic Fingerprinting by Application of rep-PCR
1Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
Methods in Molecular Medicine
|March 11, 2011
Summary
Short repetitive DNA sequences like Repetitive Extragenic Palindrome (REP) elements are common in bacteria. Their exact function remains unclear but may involve gene regulation and genome organization.
Area of Science:
- Bacterial Genetics
- Genomics
- Molecular Biology
Background:
- Short repetitive DNA sequences are prevalent in bacterial genomes.
- Repetitive Extragenic Palindrome (REP) elements and Enterobacterial Repetitive Intergenic consensus (ERIC) sequences are well-characterized examples.
- These sequences are located between genes, are not translated, and their precise function is unknown.
Purpose of the Study:
- To investigate the characteristics and potential functions of repetitive DNA sequences in bacteria.
- To describe the structure and genomic distribution of REP elements.
Main Methods:
- DNA sequence comparisons of intergenic regions.
- Identification and characterization of REP consensus sequences through sequence analysis.
Main Results:
- The REP consensus sequence is a 38-nucleotide palindrome forming a stable stem-loop structure.
- REP sequences can exist in 50-1000 copies per genome, often in clusters.
- REP elements are found in intergenic regions across various bacterial species and operons.
Conclusions:
- REP elements are widespread in eubacteria, suggesting an important role.
- Potential functions include transcription termination, mRNA stability, or chromosomal organization.
- Further research is needed to fully elucidate the function of these repetitive sequences.
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