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Related Concept Videos

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic01:26

Healthcare Associated Infections I: Iatrogenic, Exogenic and Endogenic

Healthcare-associated infections (HAIs) occur in a healthcare facility while a person receives care for another ailment. This category also includes work-related infections among healthcare staff.
HAIs significantly increase the cost of health care. Extended stays in healthcare institutions, increased disability, increased costs of medications, including specialized antibiotics, and prolonged recovery times add to the patient's expenses and the healthcare institution and funding bodies. Common...
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
Pneumonia I: Introduction01:29

Pneumonia I: Introduction

Pneumonia is an infection of the lower respiratory tract that leads to inflammation of the lung parenchyma, often resulting in the accumulation of inflammatory exudate in the alveoli and airways. Unlike the watery, low-protein fluid exudate in pulmonary edema, the exudate in this case is a thick fluid rich in immune cells, proteins, and debris produced during infection and inflammation.This impairs gas exchange and can lead to consolidation of lung tissue. The infection may be caused by a...
Pneumonia I: Introduction01:30

Pneumonia I: Introduction

Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
Healthcare Associated Infections II: Preventive Measures01:22

Healthcare Associated Infections II: Preventive Measures

Essential infection prevention measures are based on the knowledge of the infection chain, the modes of transmission in healthcare settings, and the use of the best practices in all healthcare settings. Compulsory public reporting of healthcare-associated infection rates is needed to allow individuals and the community to make informed choices regarding selecting a healthcare facility.
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Skin Diseases and Disorders01:23

Skin Diseases and Disorders

Skin is the first line of defense and encounters a variety of microbes. Some pathogenic strains are often the cause of a broad range of infections of the skin and other body systems. These conditions can affect people of all ages and may have different causes, including genetic factors, infections, autoimmune reactions, environmental factors, and lifestyle choices.
Gram-positive Staphylococcus spp. and Streptococcus spp. are responsible for many of the most common skin infections. However, many...

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Related Experiment Videos

Nosocomial infections caused by staphylococci.

A Deplano1, M J Struelens

  • 1Unite di Epidemiologie et diHygiene Hospitaliere, Laboratoire de Microbiologie, Hospital Erasma, Bruxelles, Belgium.

Methods in Molecular Medicine
|March 11, 2011
PubMed
Summary
This summary is machine-generated.

Accurate identification of staphylococci is crucial for diagnosing nosocomial infections. Current methods struggle with differentiating species, potentially leading to misidentification and ineffective treatment strategies.

Related Experiment Videos

Area of Science:

  • Clinical Microbiology
  • Bacteriology
  • Infectious Diseases

Background:

  • Staphylococcus aureus and coagulase-negative staphylococci (CNS) are common causes of hospital-acquired infections.
  • Conventional identification methods for staphylococci have limitations in species-level accuracy.
  • Misidentification can occur due to overlapping characteristics and atypical strains.

Purpose of the Study:

  • To highlight the clinical relevance of accurate CNS speciation.
  • To address the limitations of current staphylococcal identification and typing methods.
  • To emphasize the need for improved diagnostic approaches in clinical microbiology.

Main Methods:

  • Review of conventional identification techniques for staphylococci.
  • Discussion of limitations in biochemical and antimicrobial susceptibility testing.
  • Exploration of challenges in differentiating true infections from contamination.

Main Results:

  • Current methods often misidentify staphylococci, especially CNS.
  • Species like S. lugdunensis are increasingly recognized for causing severe infections.
  • Antimicrobial susceptibility patterns are unreliable for clonal delineation.

Conclusions:

  • Accurate speciation of CNS is clinically significant.
  • Improved methods are needed for reliable staphylococcal identification and typing.
  • Distinguishing true infections from contamination requires robust strain characterization.