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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Related Experiment Video

Updated: Jun 3, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
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Reverse transcriptase-polymerase chain reaction (rt-PCR).

R Kusec1

  • 1, Voncinina, Zagreb, Croatia.

Methods in Molecular Medicine
|March 11, 2011
PubMed
Summary

Reverse transcriptase polymerase chain reaction (RT-PCR) is a versatile molecular tool for analyzing RNA. It

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Reverse transcriptase polymerase chain reaction (RT-PCR) is a key molecular technique.
  • It is widely used for ribonucleic acid (RNA) analysis.

Purpose of the Study:

  • To highlight the established applications of RT-PCR.
  • To emphasize its ongoing development and new applications in research.

Main Methods:

  • RT-PCR is a powerful method for RNA detection and analysis.
  • It enables gene expression studies and identification of specific RNA transcripts.

Main Results:

  • RT-PCR is crucial in medical diagnostics for viral RNA detection.
  • It is applied in hematology and oncology for detecting chimeric transcripts.

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  • The technique is vital for research in gene expression studies.

    • Conclusions:

    • RT-PCR is a continuously evolving and expanding molecular technique.
    • Its applications are constantly increasing, particularly in gene expression research.