Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implications of 2015 guidelines on good publication practice for communicating company sponsored medical research.

Maturitas·2016
Same author

A comparison of the efficacy and tolerability of solifenacin succinate and extended release tolterodine at treating overactive bladder syndrome: results of the STAR trial.

European urology·2005
Same author

Elevated serum creatine kinase activity in a patient with acute pancreatitis.

International journal of clinical practice·2000
Same author

Historical perspectives on health. Origins of blood analysis in clinical diagnosis.

The journal of the Royal Society for the Promotion of Health·1999
Same author

Laboratory instrumentation in clinical biochemistry: an historical perspective.

Journal of the Royal Society of Medicine·1998
Same author

Telomerase activity and its inhibition in benign and malignant breast lesions.

The Journal of pathology·1997

Related Experiment Video

Updated: Jun 3, 2026

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

PCR Analysis of CD44 Variants in Tumors.

J Bolodeoku1

  • 1Department of Chemical Pathology, School of Medicine, Royal Free Hospital, London, UK.

Methods in Molecular Medicine
|March 11, 2011
PubMed
Summary

The CD44 molecule is a transmembrane glycoprotein with a protein core. It undergoes extensive glycosylation and can be further modified with chondroitin sulfate, impacting its molecular weight.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • CD44 is a cell surface glycoprotein involved in cell adhesion and signaling.
  • Its structure is complex, with variations in glycosylation and post-translational modifications.
  • Understanding CD44 structure is crucial for elucidating its diverse biological functions.

Purpose of the Study:

  • To detail the structural composition of the CD44 molecule.
  • To describe the post-translational modifications that affect CD44.
  • To provide a foundation for further research into CD44 function.

Main Methods:

  • Analysis of protein core structure.
  • Assessment of N- and O-linked glycosylation.
  • Investigation of chondroitin sulfate attachment.

More Related Videos

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications
14:14

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma
09:06

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

Published on: November 11, 2021

Related Experiment Videos

Last Updated: Jun 3, 2026

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications
14:14

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Published on: February 28, 2014

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma
09:06

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

Published on: November 11, 2021

Main Results:

  • CD44 possesses a 37-kDa protein core.
  • Extensive N- and O-linked glycosylation results in an 85-90 kDa product.
  • Chondroitin sulfate addition can yield a 180-220 kDa form.

Conclusions:

  • CD44 exhibits significant structural heterogeneity due to glycosylation and sulfation.
  • These modifications influence CD44's molecular weight and potential functions.
  • The described structural features are key to CD44's role in cellular processes.