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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 3, 2026

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

Detection of circulating solid tumor cells by reverse transcriptase polymerase chain reaction.

¹Candlelighters Children's Cancer Research Group, Cancer Medicine Research Unit, St. James' University Hospital, Leeds, UK.

Methods in Molecular Medicine

|March 11, 2011

Summary

This summary is machine-generated.

Detecting circulating tumor cells (CTCs) is crucial for understanding cancer metastasis. Early detection of these cells can predict relapse and improve patient outcomes.

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Last Updated: Jun 3, 2026

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Area of Science:

Oncology
Cancer Biology
Translational Medicine

Background:

Cancer metastasis, or the spread of disease, remains a significant clinical challenge despite treatment advances.
Metastasis involves complex tumor-host interactions, with the role of circulating tumor cells (CTCs) in secondary disease development not fully elucidated.
Successful metastasis requires cancer cells to enter and survive in circulation (e.g., peripheral blood, bone marrow).

Purpose of the Study:

To highlight the importance of detecting small numbers of circulating tumor cells (CTCs).
To underscore the potential of CTC detection as a tool for predicting cancer recurrence and relapse.
To explore how CTC detection can lead to improved clinical outcomes through targeted therapies.

Main Methods:

The abstract does not detail specific experimental methods but focuses on the clinical implications of detecting circulating tumor cells.
It emphasizes the need for sensitive detection methods to identify rare CTCs in peripheral blood or bone marrow.
The study implies a focus on the biological and clinical significance of CTCs.

Main Results:

Less than 0.01% of circulating tumor cells are thought to successfully establish metastatic colonies.
Detecting even small numbers of CTCs is vital for evaluating their role in disease progression.
The presence and characteristics of CTCs can serve as indicators of disease recurrence.

Conclusions:

Detecting circulating tumor cells (CTCs) offers a promising avenue for predicting cancer recurrence and relapse.
Early identification of micrometastatic disease or preclinical relapse via CTCs can guide treatment decisions.
This approach holds the potential for improved patient outcomes through timely and personalized therapeutic interventions.