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Single cell PCR : theory, practice, and clinical applications

I Findlay1

  • 1Centre Reproduction, Growth, and Development, Institute of Pathology, Algernon Firth Building, University of Leeds, UK.

Methods in Molecular Medicine
|March 11, 2011
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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