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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Feb 20, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Flow cytometry.

¹Institute for Immunology, University of Heidelberg, Germany.

Methods in Molecular Medicine

|March 11, 2011

Summary

This summary is machine-generated.

Flow cytometry is a quantitative, rapid technique that analyzes individual cells for multiple characteristics. This method is crucial for cell analysis, sorting, and defining cell types in complex biological samples.

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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

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Last Updated: Feb 20, 2026

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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Area of Science:

Cell Biology
Immunology
Biotechnology

Background:

Flow cytometry is a widely adopted technique for analyzing cellular characteristics.
It measures fluorescence intensity from labeled antibodies or ligands binding to cell-associated molecules.

Purpose of the Study:

To provide a comprehensive overview of the flow cytometry technique.
To highlight its quantitative, rapid, and reproducible nature for cell analysis.

Main Methods:

Utilizes fluorescently labeled antibodies or ligands to detect specific cell-associated molecules.
Analyzes individual cells for multiple parameters simultaneously, including size, viability, and molecule expression.
Enables electronic cell sorting for isolating specific cell populations.

Main Results:

Flow cytometry allows for quantitative, high-throughput analysis (>10,000 cells/min).
It facilitates simultaneous measurement of various cellular parameters like size, viability, and activation.
The technique supports the characterization of heterogeneous cell populations and purity analysis.

Conclusions:

Flow cytometry is a versatile tool for analyzing intracellular and surface molecule expression on a per-cell basis.
Applications include cell type characterization, purity assessment, viability determination, apoptosis detection, cell cycle analysis, and calcium flux measurement.
Its quantitative and multi-parametric capabilities make it essential in various biological research fields.