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RP-HPLC method for simultaneous determination of butenafine hydrochloride and betamethasone dipropionate in a cream
Suryakant D Bhosale1, Sadhana J Rajput
1The Maharaja Sayajirao University of Baroda, Quality Assurance Laboratory, Pharmacy Department, Faculty of Technology and Engineering, Vadodara-390 001, India. sd_bhosale73@rediffmail.com
Abstract:
An RP-HPLC method has been developed for the simultaneous determination of butenafine hydrochloride and betamethasone dipropionate on an Inertsil C18 column (250 x 4.6 mm id) using a mobile phase gradient consisting of methanol and water at a flow rate of 1 mL/min. Detection was carried out at 254 nm. Retention times of betamethasone dipropionate and butenafine hydrochloride were 4.82 (+/- 0.80) and 16.18 (+/- 0.17) min, respectively. The method was validated with respect to specificity, linearity, accuracy, precision, ruggedness, and robustness. This method is simple, precise, and sensitive, and applicable for the simultaneous quantification of butenafine hydrochloride and betamethasone dipropionate in a cream formulation.
