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Related Experiment Video

Updated: Jul 21, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
07:33

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Published on: January 4, 2014

Characterization of infectious transcripts from a potato virus X cDNA clone.

C Hemenway1, J Weiss, K O'Connell

  • 1Monsanto Company, St. Louis, Missouri 63198.

Virology
|April 1, 1990
PubMed
Summary

Researchers created an infectious clone of potato virus X (PVX) from complementary DNA (cDNA). This breakthrough enables detailed studies into the molecular mechanisms of PVX infection in plants.

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Last Updated: Jul 21, 2026

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Area of Science:

  • Plant virology
  • Molecular biology
  • Molecular cloning

Background:

  • Potato virus X (PVX) is a significant plant pathogen.
  • Understanding PVX infection requires tools for genetic manipulation.

Purpose of the Study:

  • To construct a full-length infectious complementary DNA (cDNA) clone of PVX.
  • To enable molecular analysis of PVX infection.

Main Methods:

  • Construction of a full-length PVX cDNA clone.
  • In vitro transcription using a bacteriophage T7 promoter.
  • Inoculation of transcripts onto local lesion host plants (Chenopodium amaranticolor).
  • Electron microscopy for virus particle visualization.
  • Infectivity assays of progeny virus.

Main Results:

  • Transcripts from the PVX cDNA clone (pMON 8660) were infectious.
  • Infectivity was approximately 0.2% of authentic PVX RNA.
  • Virus particles and aggregates in lesions matched authentic PVX infections.
  • Progeny virus from cDNA clone infections was as infectious as from authentic RNA.

Conclusions:

  • Infectious PVX cDNA clones provide a valuable tool for molecular studies.
  • This facilitates research into the mechanisms of PVX pathogenesis.
  • Enables future investigations into PVX-host interactions.