Related Experiment Video
Updated: Jun 3, 2026

Hydrogen Production and Utilization in a Membrane Reactor
Published on: March 10, 2023
Dehydrogenase activity in association with poised potential during biohydrogen production in single chamber microbial
S Venkata Mohan1, M Lenin Babu
1Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Hyderabad 500 607, India. vmohan_s@yahoo.com
Abstract:
Variation in the dehydrogenase (DH) activity and its simultaneous influence on hydrogen (H2) production, substrate degradation rate (SDR) and volatile fatty acid (VFA) generation was investigated with respect to varying poised potential in single chambered membrane-less microbial electrolysis cell (MEC) using anaerobic consortia as biocatalyst. Poised potential showed significant influence on H2 production and DH activity. Maximum H2 production was observed at 1.0V whereas the control system showed least H2 production among the experimental variations studied. DH activity was observed maximum at 0.6V followed by 0.8, 0.9 and 1.0V, suggests the influence of poised potential on the microbial metabolism. Almost complete degradation of substrate was observed in all the experimental conditions studied irrespective of the applied potential. Experimental data was also analysed employing multiple regression analysis and 3D-surface plots to find out the best theoretical poised potential for maximum H2 production and DH activity.
More Related Videos
Related Concept Videos
Microbial Fuel Cells
Standard Electrode Potentials
Chemiosmosis
Electron Transport Chain
The electron transport chain involves a series of protein complexes on the inner mitochondrial membrane that undergo a series of redox reactions. At the end of this chain, the electrons reduce...
Electrolysis
Processes at Electrodes
Concentration Cells
Consider the following voltaic cell:
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
