Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Biofuels

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

Lipid Catabolism

Lipid Catabolism

Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...

Production of Alcohol

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

Microbial Bioremediation of Plastics

Microbial Bioremediation of Plastics

Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Biocatalytic processes in ionic liquids: a green platform for a sustainable future.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2026

Same author

Closing the Loop on Polyurethane Foam Waste: Challenges, Emerging Technologies, and the Road to Sustainable Circularity.

ACS sustainable chemistry & engineering·2026

Same author

Biocatalytic Epoxidation Pathway of Oils in Sponge-Like Ionic Liquids Toward CO<sub>2</sub>-Derived Cyclic Carbonate Synthesis.

ChemSusChem·2026

Same author

Spain's Chemistry Turns Over a New Green Leaf.

ChemSusChem·2025

Same author

On the Hydrolytic Depolymerization of Polyurethane Foam Wastes by Ionic Liquids.

Molecules (Basel, Switzerland)·2025

Same author

How to Easily Depolymerize Polyurethane Foam Wastes by Superbase Catalysts in Ionic Liquids Below 100 °C.

Angewandte Chemie (International ed. in English)·2024

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Published on: August 10, 2016

A recyclable enzymatic biodiesel production process in ionic liquids.

Teresa De Diego¹, Arturo Manjón, Pedro Lozano

¹Department of Biochemistry and Molecular Biology B and Immunology, Faculty of Chemistry, University of Murcia, P.O. Box 4021, E-30100 Murcia, Spain.

Bioresource Technology

|March 12, 2011

Summary

This summary is machine-generated.

Immobilized lipase B in ionic liquids efficiently produces biodiesel, allowing easy separation and recycling of the enzyme and solvent for greener production.

More Related Videos

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Published on: April 19, 2024

Related Experiment Videos

Last Updated: Jun 3, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Published on: August 10, 2016

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Ultrasonic-Assisted Preparation of Biodiesel Products from Vegetable Oils

Published on: April 19, 2024

Area of Science:

Biocatalysis
Green Chemistry
Renewable Energy

Background:

Biodiesel production often faces challenges with enzyme stability and product separation.
Ionic liquids offer unique properties for enzymatic reactions but require efficient recovery methods.

Purpose of the Study:

To evaluate immobilized Candida antarctica lipase B in long alkyl-chain ionic liquids for biodiesel synthesis.
To assess the operational stability and recyclability of the enzyme and ionic liquid system.
To demonstrate a green and efficient method for biodiesel production and purification.

Main Methods:

Immobilization of Candida antarctica lipase B.
Biodiesel synthesis using enzymatic transesterification in specific ionic liquids ([C(16)MIM][NTf(2)] and [C(18)MIM][NTf(2)]).
Analysis of reaction kinetics, enzyme activity, and operational stability.
Development of a three-phase separation technique for product extraction and component recycling.

Main Results:

The immobilized lipase B exhibited excellent synthetic activity and operational stability in the tested ionic liquids.
The ionic liquids formed homogeneous systems initially and separated into three phases post-reaction.
This facilitated straightforward separation of biodiesel and enabled efficient recycling of both the enzyme and ionic liquid.
The process demonstrated high potential for environmentally friendly biodiesel production.

Conclusions:

Immobilized Candida antarctica lipase B in long alkyl-chain ionic liquids is a highly effective biocatalyst for sustainable biodiesel production.
The developed three-phase system allows for facile product recovery and reuse of biocatalyst and solvent, aligning with green chemistry principles.
This approach offers significant advantages for industrial-scale, eco-friendly biodiesel manufacturing.