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TREM-1 interaction with the LPS/TLR4 receptor complex.

Rob J W Arts¹, Leo A B Joosten, Charles A Dinarello

¹Radboud University Nijmegen Medical Center; and Nijmegen Institute for Infection, Inflammation and Immunity (N4i), Nijmegen, The Netherlands.

European Cytokine Network

|March 12, 2011

Summary

This summary is machine-generated.

Triggering receptor expressed on myeloid cells 1 (TREM-1) interacts with toll-like receptor 4 (TLR4), amplifying inflammation. Blocking either receptor complex component reduced inflammatory responses, suggesting a functional link in immune activation.

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Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation

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Published on: September 7, 2010

Area of Science:

Immunology
Molecular Biology

Background:

Triggering receptor expressed on myeloid cells 1 (TREM-1) is an activating receptor on neutrophils and monocytes.
TREM-1 amplifies inflammation triggered by pattern-recognition receptors.

Purpose of the Study:

To investigate the interaction between TREM-1 and the toll-like receptor 4 (TLR4) complex.
To elucidate the functional consequences of this interaction on inflammatory responses.

Main Methods:

Utilized blocking anti-TREM-1 antibodies to inhibit LPS-induced TNF-α production.
Employed a specific TLR4 inhibitor to assess its effect on TREM-1 cross-linking.
Investigated TLR4-TREM1 co-localization in human neutrophils.

Main Results:

Blocking anti-TREM-1 antibodies specifically inhibited lipopolysaccharide (LPS)-induced TNF-α production.
TLR4 inhibition down-regulated the effects of TREM-1 cross-linking.
Demonstrated TLR4 and TREM-1 co-localization in human neutrophils.

Conclusions:

TREM-1 interacts with the TLR4 receptor complex, potentially as a component.
This interaction is functionally significant for inflammatory responses.
Biological effects of TREM-1 may be mediated through its association with the TLR4/LPS-receptor complex.