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Updated: Jun 3, 2026

Optimization of Crystal Growth for Neutron Macromolecular Crystallography
Published on: March 13, 2021
Influence of temperature during crystallization setup on precipitate formation and crystal shape of a
Xenia Bogdanović1, Winfried Hinrichs
1Department of Molecular Structural Biology, Institute for Biochemistry, Ernst-Moritz-Arndt University, Greifswald, Germany.
Abstract:
It is well known that protein crystallization is affected by several different parameters such as the composition of the reservoir solution, the protein concentration, the pH and the temperature. An effect of different temperatures during setup of crystallization experiments was observed for a metalloendopeptidase (AsaP1(E294A)). Spontaneous protein precipitation was reduced and the crystal shape could be improved by decreasing the temperature during crystallization setup.
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