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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Updated: Jun 3, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants
12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

In situ hybridization.

X J Yang1

  • 1Department of Ophthalmology and Neurobiology, Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, CA.

Methods in Molecular Medicine
|March 12, 2011
PubMed
Summary
This summary is machine-generated.

In situ hybridization visualizes RNA within cells using labeled probes. This technique is valuable for locating RNA when protein antibodies are unavailable.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • In situ hybridization (ISH) is a powerful molecular biology technique.
  • It enables the detection and localization of specific nucleic acid sequences within their cellular context.
  • ISH is particularly useful for studying gene expression patterns at the cellular and subcellular levels.

Purpose of the Study:

  • To describe the principles and applications of in situ hybridization.
  • To highlight the utility of ISH for RNA localization.
  • To emphasize ISH as an alternative to immunocytochemical staining when antibodies are unavailable.

Main Methods:

  • Utilizes labeled nucleic acid probes that specifically bind to complementary cellular RNAs.
  • Involves the detection of these labeled probes within fixed cells, tissue sections, or whole mount tissues.
  • Relies on the principle of nucleic acid hybridization for target identification.

Main Results:

  • Successfully demonstrates the cellular and subcellular localization of transcribed RNA.
  • Provides visual evidence of RNA distribution within various biological samples.
  • Confirms the effectiveness of ISH for RNA detection.

Conclusions:

  • In situ hybridization is a versatile and essential technique for RNA analysis.
  • It offers precise localization of RNA transcripts.
  • ISH serves as a critical tool in molecular and cell biology research, especially in the absence of specific protein antibodies.