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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Related Experiment Video

Updated: Jun 3, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
09:56

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

Generation of knockout animal models.

T M Redmond1

  • 1NEI-LRCMB, NIH, Bethesda, Maryland.

Methods in Molecular Medicine
|March 12, 2011
PubMed
Summary

Researchers create genetically modified animals to study gene function and human diseases. This technique is crucial for understanding vision disorders and other genetic conditions by observing the effects of specific gene loss in vivo.

Area of Science:

  • Biotechnology
  • Genetics
  • Ophthalmology

Background:

  • Pluripotent stem cell culture and gene editing enable the creation of genetically modified organisms.
  • Chimeric animal models are vital tools for in vivo research across various scientific disciplines.
  • These models offer unique insights into the physiological and structural consequences of gene loss.

Purpose of the Study:

  • To investigate the in vivo effects of targeted gene mutations.
  • To leverage gene-edited cell lines for the generation of chimeric animals.
  • To explore the utility of these models in ophthalmology and vision research.

Main Methods:

  • Culturing pluripotent stem cell lines.
  • Introducing targeted gene mutations into these cell lines.

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Related Experiment Videos

Last Updated: Jun 3, 2026

Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation

Published on: August 26, 2025

Generation of Genetically Modified Mice through the Microinjection of Oocytes
10:19

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

  • Generating chimeric animals from mutant cell lines.

    • Main Results:

    • Successful generation of chimeric animals with specific gene deficiencies.
    • Observation of biochemical, physiological, and structural changes resulting from gene loss.
    • Identification of potential insights into human diseases linked to gene mutations.

    Conclusions:

    • Genetically engineered chimeric animals are powerful tools for studying gene function and loss-of-function phenotypes.
    • This methodology significantly advances vision research and the understanding of genetic disorders.
    • The approach provides a platform for uncovering disease mechanisms and potential therapeutic targets.