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Published on: May 2, 2025
[Experiment studies on growth and proliferation of Hep-2 cells by silence of TRF2 gene]
Luyan Jiao1, Yong Xu, Zezhang Tao
1Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Objective:
To investigate the effect of RNA interference silencing telomere repeat factor 2 by observing Hep-2 cells' proliferation and apoptosis in larynx carcinoma cell line.
Method:
A recombinant plasmid containing a single shRNA (shTRF2) was constructed. The expression of TRF2 gene was detected by PCR and cell proliferation was examined using CCK-8. Hep-2 cells' apoptosis was detected by flow cytometer (FCM).
Result:
After treatment with shTRF2, the expression of TRF2 was distinctively depressed, and Hep-2 cells proliferation was obviously inhibited. Compared with control and negative group, cells with treatment of RNAi exhibited significantly more apoptosis.
Conclusion:
Using RNA interference technique to silence TRF2 gene is effective on inhibiting cancer cells' proliferation and help to induce cancer cells' apoptosis.
Insights
RNA interference effectively silenced the telomere repeat factor 2 (TRF2) gene in larynx carcinoma cells. This inhibition suppressed cell proliferation and promoted apoptosis, offering a potential therapeutic strategy.
Area of Science:
- Molecular Biology
- Oncology
Context:
- Larynx carcinoma is a significant global health concern.
- Telomere repeat factor 2 (TRF2) plays a crucial role in telomere maintenance and has been implicated in cancer progression.
- Understanding TRF2's role is vital for developing targeted cancer therapies.
Purpose:
- To investigate the therapeutic potential of silencing the TRF2 gene in larynx carcinoma cells using RNA interference (RNAi).
- To assess the impact of TRF2 gene silencing on Hep-2 cell proliferation and apoptosis.
Summary:
- A recombinant plasmid with short hairpin RNA targeting TRF2 (shTRF2) was constructed and introduced into Hep-2 cells.
- TRF2 gene expression was significantly reduced following shTRF2 treatment, as confirmed by PCR.
- Cell proliferation was markedly inhibited, and apoptosis was significantly induced in Hep-2 cells treated with shTRF2 compared to control groups, as determined by CCK-8 and flow cytometry, respectively.
Impact:
- RNA interference-mediated silencing of TRF2 demonstrates efficacy in inhibiting larynx carcinoma cell proliferation.
- This approach effectively induces apoptosis in cancer cells, suggesting a promising avenue for novel cancer treatment strategies.
- The findings contribute to the understanding of TRF2's oncogenic function and its potential as a therapeutic target.
