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Updated: Jun 3, 2026
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Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
Published on: August 16, 2018
Development of expression vectors based on pepino mosaic virus.
Raquel N Sempere1, Pedro Gómez, Verónica Truniger
1Departamento de Biología del Estrés y Patología Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS)- CSIC, PO Box 164, 30100 Espinardo, Murcia, Spain. m.aranda@cebas.csic.es.
Plant Methods
|March 15, 2011
Summary
Researchers developed a stable plant expression vector using Pepino mosaic virus (PepMV). This novel tool efficiently produces recombinant proteins and aids in gene function studies.
Area of Science:
- Plant virology
- Molecular biology
- Biotechnology
Background:
- Plant viruses serve as effective expression vectors for rapid, large-scale recombinant protein production in plants.
- Pepino mosaic virus (PepMV) is a promising candidate due to high accumulation and mild symptoms.
Purpose of the Study:
- To construct a stable and efficient plant expression vector based on Pepino mosaic virus (PepMV).
- To evaluate different strategies for vector development and optimize transgene expression.
Main Methods:
- Construction of agroinfectious clones from two PepMV genotypes.
- Exploration of coat protein (CP) replacement, CP subgenomic promoter (SGP) duplication, and foot-and-mouth disease virus (FMDV) 2A peptide fusion strategies.
- Assessment of vector stability and recombinant protein accumulation (GFP).
Main Results:
- CP replacement and duplicated SGP vectors showed poor systemic movement and transgene instability.
- Fusion protein strategy using FMDV 2A peptide resulted in a stable vector.
- Achieved high accumulation of Green Fluorescent Protein (GFP) (0.2-0.4 g/kg leaf tissue).
- Demonstrated PepMV vector utility for virus-induced gene silencing.
Conclusions:
- A stable PepMV-based expression vector was successfully generated using an FMDV 2A peptide fusion.
- This novel tool facilitates recombinant protein expression and gene function analysis in plants.
- The study elucidated virus replication and movement requirements.
