Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Published on: May 30, 2016

Vascularization is the key challenge in tissue engineering.

Esther C Novosel¹, Claudia Kleinhans, Petra J Kluger

¹University of Stuttgart, Institute for Interfacial Engineering, Nobelstrasse 12, Stuttgart, Germany.

Advanced Drug Delivery Reviews

|March 15, 2011

Summary

Engineering vascularized tissues in vitro faces challenges due to insufficient blood vessel networks. This review compares endothelial cell-driven neoangiogenesis and scaffold-based methods for creating functional vascularization in engineered tissues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants.

Macromolecular bioscience·2024

Same author

Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat.

Nature communications·2024

Same author

Robot-based 6D bioprinting for soft tissue biomedical applications.

Engineering in life sciences·2024

Same author

Bioprinting of 3D Adipose Tissue Models Using a GelMA-Bioink with Human Mature Adipocytes or Human Adipose-Derived Stem Cells.

Gels (Basel, Switzerland)·2022

Same author

Gellan Gum Is a Suitable Biomaterial for Manual and Bioprinted Setup of Long-Term Stable, Functional 3D-Adipose Tissue Models.

Gels (Basel, Switzerland)·2022

Same author

Eclectic characterisation of chemically modified cell-derived matrices obtained by metabolic glycoengineering and re-assessment of commonly used methods.

RSC advances·2022

Area of Science:

Biomedical Engineering
Tissue Engineering
Regenerative Medicine

Background:

Vascularization is crucial for engineered tissues, mimicking in vivo blood vessel networks.
Lack of sufficient vascularization limits the viability and function of engineered tissues.
Endothelial cells form tubular networks essential for tissue supply.

Purpose of the Study:

To review current strategies for creating vascularized tissues in vitro.
To compare different approaches for generating functional blood vessel systems.
To discuss future directions in vascularized tissue engineering.

Main Methods:

Review of endothelial cell-based neoangiogenesis techniques.
Comparison of prevascularization and biomolecule/cell-guided vessel formation.

More Related Videos

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Published on: August 28, 2014

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Related Experiment Videos

Last Updated: Jun 3, 2026

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Published on: May 30, 2016

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

A Full Skin Defect Model to Evaluate Vascularization of Biomaterials In Vivo

Published on: August 28, 2014

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids

Published on: August 11, 2017

Analysis of scaffold-based techniques using natural and synthetic matrices.

Main Results:

Neoangiogenesis strategies leverage endothelial cell self-assembly and growth factors.
Scaffold-based methods utilize pre-existing vessels in natural materials or engineered synthetic matrices.
Both approaches have distinct advantages and limitations for in vitro vascularization.

Conclusions:

Effective vascularization remains a key challenge in tissue engineering.
A combination of cell-based and scaffold-based strategies may offer future solutions.
Further research is needed to optimize methods for creating clinically relevant vascularized tissues.