Prospective isolation of human MSC
Abhishek Harichandan1, Hans-Jörg Bühring
1University Clinic of Tübingen, Department of Internal Medicine II, Division of Haematology, Immunology, Oncology, Rheumatology, and Pulmonology, Laboratory for Stem Cell Research, Otfried-Müller-Str. 10, 72076 Tübingen, Germany.
Best Practice & Research. Clinical Haematology
|March 15, 2011
Summary
Conventional isolation of mesenchymal stem cells (MSC) faces challenges due to contamination and timing issues. This study explores novel surface markers for more precise antibody-based isolation of bone marrow-derived MSC.
Area of Science:
- Stem Cell Biology
- Immunology
Background:
- Mesenchymal stem cells (MSC) are conventionally isolated by plastic adherence, a method prone to contamination from other cells and secreted factors.
- Current isolation protocols are limited by the timing of non-adherent cell removal, potentially excluding late-adhering MSC subsets or allowing unwanted cell influence.
Purpose of the Study:
- To address limitations of conventional MSC isolation methods.
- To identify and summarize established and novel surface markers for prospective antibody-based isolation of MSC.
Main Methods:
- Review of literature on commonly used surface markers for MSC isolation.
- Identification of new potential surface markers for antibody-based isolation strategies.
Main Results:
- Conventional MSC isolation relies on plastic adherence, which is suboptimal.
- Surface marker-based isolation offers a more precise alternative to plastic adherence.
- Several established and novel surface markers are discussed for improved MSC isolation.
Conclusions:
- Prospective isolation of MSC using surface markers circumvents the limitations of conventional plastic adherence methods.
- Antibody-based isolation targeting specific surface markers provides a more reliable approach for obtaining pure MSC populations.
- This review highlights key markers for enhancing the isolation of bone marrow-derived MSC.
