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Isolation of Atrial Myocytes from Adult Mice
Published on: July 25, 2019
Expression of B-type natriuretic peptide forms in ischemic human hearts
Raquel Cortés1, Esther Roselló-Lletí, Miguel Rivera
1Cardiocirculatory Unit, Research Center, Hospital Universitario La Fe, Valencia, Spain.
International Journal of Cardiology
|March 15, 2011
Summary
Pro-B-type natriuretic peptide (proBNP) and its related peptides (BNP, NT-proBNP) and GATA-4 are elevated in ischemic cardiomyopathy (ICM) heart tissue. These biomarkers show significant correlations, suggesting their potential role in ICM.
Area of Science:
- Cardiovascular Research
- Biomarker Discovery
- Molecular Cardiology
Background:
- Natriuretic peptides (NPs) like BNP and NT-proBNP are established biomarkers for heart failure.
- The precursor proBNP and the transcription factor GATA-4 have also been implicated in cardiac function and disease.
- Understanding their expression and correlation in ischemic conditions is crucial for diagnostic and therapeutic advancements.
Purpose of the Study:
- To quantify proBNP, BNP, NT-proBNP, and GATA-4 in human cardiac tissue.
- To investigate the correlations among these biomarkers.
- To determine the influence of ischemic etiology on their levels.
Main Methods:
- Western blot analysis for protein quantification.
- Quantitative real-time PCR (RT-PCR) for mRNA expression analysis.
- Analysis of 33 human heart samples (ischemic and control).
Main Results:
- ProBNP protein levels were significantly higher than BNP and NT-proBNP in ischemic hearts.
- ProBNP mRNA expression was elevated in ischemic cardiomyopathy (ICM) samples.
- Strong correlations were observed among proBNP, BNP, NT-proBNP, and GATA-4 in ischemic tissues.
- All four biomarkers (proBNP, BNP, NT-proBNP, GATA-4) showed increased tissue protein levels in ICM compared to controls.
Conclusions:
- Human heart tissue exhibits higher proBNP protein levels than BNP and NT-proBNP.
- Increased proBNP mRNA expression is associated with ICM.
- Natriuretic peptides and GATA-4 show significant correlations in cardiac tissue.
- Ischemic cardiomyopathy is characterized by elevated tissue levels of proBNP, BNP, NT-proBNP, and GATA-4.
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