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Small-molecule probe using dual signals to monitor leucine aminopeptidase activity

Hey Young Yoon1, So Hee Shim, Luck Ju Baek

  • 1Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Republic of Korea.

Bioorganic & Medicinal Chemistry Letters
|March 15, 2011
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...