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Updated: Jun 3, 2026
10:24
Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation
Published on: August 24, 2018
New biotech applications from evolved D-amino acid oxidases
Loredano Pollegioni1, Gianluca Molla
1Dipartimento di Biotecnologie e Scienze Molecolari, Università degli Studi dell'Insubria, Varese, Italy. loredano.pollegioni@uninsubria.it
Trends in Biotechnology
|March 15, 2011
Summary
D-Amino acid oxidase (DAAO) is a versatile flavoenzyme. Protein engineering enhances DAAO
Area of Science:
- Biochemistry
- Enzymology
- Protein Engineering
Background:
- D-Amino acid oxidase (DAAO) is a flavoenzyme catalyzing stereospecific oxidative deamination of D-amino acids.
- This reaction produces alpha-keto acids, ammonia, and hydrogen peroxide.
- Recent studies highlight DAAO's functional plasticity, increasing research interest.
Purpose of the Study:
- To review recent advancements in the utilization and engineering of D-Amino acid oxidase.
- To explore the biotechnological potential of DAAO as an oxidative biocatalyst.
Main Methods:
- Literature review of recent developments in DAAO research.
- Analysis of protein engineering strategies applied to DAAO.
- Examination of DAAO applications in biocatalysis, biosensing, and herbicide resistance.
Main Results:
- Protein engineering has successfully modified DAAO's substrate specificity, oxygen affinity, cofactor binding, stability, and oligomeric state.
- DAAO is effectively utilized as a biocatalyst for resolving racemic amino acid mixtures.
- DAAO serves as a valuable tool in biosensing and has been identified as a novel mechanism for herbicide resistance.
Conclusions:
- DAAO exhibits remarkable functional plasticity, making it a highly adaptable biocatalyst.
- Engineered DAAO holds significant promise for diverse biotechnological applications.
- Further research into DAAO's capabilities will likely unlock new innovative uses.
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