In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production

Jan Erik Rau1, Ulrich Fischer

  • 1Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobener Strasse, D-28359 Bremen, Germany. jerau@uni-bremen.de

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Oxidations of Aldehydes and Ketones to Carboxylic Acids01:15

Oxidations of Aldehydes and Ketones to Carboxylic Acids

Oxidation of aldehydes and ketones results in the formation of carboxylic acids. Aldehydes, bearing hydrogen next to the carbonyl group, are easily oxidized compared to ketones. This is because an aldehydic proton can easily be abstracted during oxidation.
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...