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Updated: Jun 3, 2026

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
Published on: November 5, 2013
In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production
1Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobener Strasse, D-28359 Bremen, Germany. jerau@uni-bremen.de
Abstract:
A simple and practicable method for in-gel detection of bacterial produced L-amino acid oxidases (L-AAOs) after non-denaturing SDS-PAGE based on the visualisation of occurring hydrogen peroxide production is described. Advantages of this screening method for L-AAOs are the determination of their numbers and approximate molecular weights additionally in one approach.
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