Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidemiology and clinical management of 1072 dogs with diabetes mellitus in a UK diabetes register.

Companion animal health and genetics·2026
Same author

Complications and outcomes of temporary tracheostomy in 24 cats.

The Journal of small animal practice·2026
Same author

European Network for Optimization of Veterinary Antimicrobial Therapy (ENOVAT) 2025 guidelines for surgical antimicrobial prophylaxis in dogs and cats.

The Journal of small animal practice·2025
Same author

Exploration of the potential utility of the luciferase immunoprecipitation system (LIPS) assay for the detection of anti-leptospira antibodies in dogs.

Veterinary immunology and immunopathology·2023
Same author

Endoscopic treatment of acute oropharyngeal stick injuries in dogs: 46 cases (2010-2020).

The Journal of small animal practice·2023
Same author

Comparative Pharmacokinetics and Local Tolerance of Tenofovir Alafenamide (TAF) From Subcutaneous Implant in Rabbits, Dogs, and Macaques.

Frontiers in pharmacology·2022

Related Experiment Video

Updated: Jun 3, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

Targeted knockdown of canine KIT (stem cell factor receptor) using RNA interference.

R C Elders1, A Holder, S J Baines

  • 1Department of Veterinary Clinical Sciences, Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL97TA, UK.

Veterinary Immunology and Immunopathology
|March 15, 2011
PubMed
Summary

Small inhibitory RNA (siRNA) effectively reduced canine KIT mRNA, including specific mutant versions driving aggressive mast cell tumors. This RNA interference approach shows promise for targeted cancer therapy in dogs.

More Related Videos

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
12:19

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

Published on: February 12, 2020

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
08:27

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Published on: April 10, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA
12:19

Stable Knockdown of Genes Encoding Extracellular Matrix Proteins in the C2C12 Myoblast Cell Line Using Small-Hairpin (sh)RNA

Published on: February 12, 2020

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
08:27

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Published on: April 10, 2018

Area of Science:

  • Veterinary Oncology
  • Molecular Biology
  • RNA Interference

Background:

  • Canine mast cell tumors frequently harbor KIT mutations, leading to c-kit receptor overactivation and aggressive disease.
  • Targeting these mutations offers a potential therapeutic strategy for canine cancer.

Purpose of the Study:

  • To investigate the efficacy of small interfering RNA (siRNA) in specifically knocking down canine KIT mRNA.
  • To assess the potential of siRNA to target both wild-type and mutant KIT sequences.

Main Methods:

  • Canine KIT, beta-2 microglobulin (B2M), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequences were cloned into a reporter vector.
  • siRNA molecules targeting specific canine genes were co-transfected into Chinese hamster ovary (CHO) cells.
  • Reporter gene expression was quantified using a dual luciferase assay to measure knockdown efficiency.

Main Results:

  • siRNA demonstrated significant knockdown of canine housekeeping genes B2M (72.0%) and GAPDH (94.5%).
  • A KIT-specific siRNA achieved 90.8% knockdown of a KIT exon 2 reporter construct.
  • An siRNA targeting a KIT exon 11 mutation selectively knocked down the mutant construct (93.1%) without affecting the wild-type construct.

Conclusions:

  • RNA interference is a viable method for inhibiting canine KIT mRNA expression.
  • siRNA technology can be developed to selectively target oncogenic KIT mutations in canine mast cell tumors.