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Published on: December 23, 2022
Complete genome sequence of the hemotrophic Mycoplasma suis strain KI3806
Joern Oehlerking1, Michael Kube, Kathrin M Felder
1Institute of Veterinary Bacteriology, Vetsuisse Faculty, University Zurich, 8057 Zurich, Switzerland.
Abstract:
Mycoplasma suis, a member of the hemotrophic mycoplasma (HM) group, parasitize erythrocytes of pigs. Increasing evidence suggests that M. suis is also a zoonotic agent. Highly pathogenic strains of M. suis (e.g., M. suis KI3806) have been demonstrated to invade erythrocytes. This complete sequenced and manually annotated genome of M. suis KI3806 is the first available from this species and from the HM group. The DNA was isolated from blood samples of experimentally infected pigs due to the lack of an in vitro cultivation system. The small circular chromosome of 709,270 bp, encoding an unexpectedly high number of hypothetical proteins and limited transport and metabolic capacities, could reflect the unique lifestyle of HM on the surface of erythrocytes.
Insights
This study presents the first complete genome sequence for Mycoplasma suis KI3806, a pathogenic swine hemotrophic mycoplasma. The genome reveals insights into the unique lifestyle of this emerging zoonotic pathogen.
Area of Science:
- Veterinary Microbiology
- Genomics
- Infectious Diseases
Background:
- Mycoplasma suis is a hemotrophic mycoplasma (HM) that infects pig erythrocytes.
- Evidence suggests Mycoplasma suis may be a zoonotic agent.
- Highly pathogenic strains, like M. suis KI3806, can invade erythrocytes.
Purpose of the Study:
- To provide the first complete, manually annotated genome sequence of Mycoplasma suis KI3806.
- To analyze the genomic features of a highly pathogenic M. suis strain.
- To gain insights into the unique lifestyle of hemotrophic mycoplasmas.
Main Methods:
- DNA isolation from blood samples of experimentally infected pigs.
- Whole-genome sequencing.
- Manual genome annotation.
Main Results:
- The complete genome sequence of M. suis KI3806 was determined (709,270 bp).
- The genome contains a high number of hypothetical proteins.
- Limited transport and metabolic gene content was observed.
Conclusions:
- The M. suis KI3806 genome provides a foundational resource for this species and the HM group.
- Genomic features suggest adaptations for an erythrocyte-surface-bound lifestyle.
- Further research can explore M. suis pathogenicity and zoonotic potential.
