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Related Experiment Video

Updated: Jun 3, 2026

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

The synaptic proteome during development and plasticity of the mouse visual cortex.

Martijn Dahlhaus1, Ka Wan Li, Roel C van der Schors

  • 1Netherlands Institute for Neuroscience, an institute of the Royal Netherlands Academy of Arts and Sciences, Amsterdam, The Netherlands.

Molecular & Cellular Proteomics : MCP
|March 15, 2011
PubMed
Summary

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Synaptic protein levels change during critical periods of brain development. This study reveals novel proteins and pathways involved in maintaining or restricting neocortex plasticity.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • The neocortex exhibits heightened plasticity during development, crucial for learning and skill acquisition.
  • Synaptic molecular changes underpin persistent modifications in neuronal connectivity and plasticity.

Purpose of the Study:

  • To quantify synaptic protein levels during critical periods of mouse visual cortex plasticity.
  • To investigate the regulation of the critical period and its molecular underpinnings.

Main Methods:

  • Utilized isobaric tag for relative and absolute quantification (iTRAQ) to measure 467 synaptic proteins.
  • Employed a well-established mouse visual cortex plasticity model.
  • Manipulated plasticity via monocular deprivation and dark rearing.

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Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
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Published on: September 17, 2011

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice
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Published on: June 22, 2021

Related Experiment Videos

Last Updated: Jun 3, 2026

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Published on: December 15, 2016

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
08:30

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Published on: September 17, 2011

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice
06:33

Implantation of a Cranial Window for Repeated In Vivo Imaging in Awake Mice

Published on: June 22, 2021

Main Results:

  • Monocular deprivation during the critical period upregulated kinases, actin-cytoskeleton, and endocytosis proteins.
  • Post-critical period, proteins for vesicle release and tubulin/septin-cytoskeletons increased, while actin regulators decreased.
  • Dark rearing into adulthood partially prevented these changes and increased G-proteins and protein kinase A.

Conclusions:

  • Dark rearing may activate pathways maintaining visual cortex plasticity, rather than just delaying development.
  • Identified novel candidate plasticity proteins and signaling pathways regulating synaptic plasticity during development and adulthood.