Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Carbohydrate Metabolism

Carbohydrate Metabolism

Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...

Carbohydrate Metabolism

Carbohydrate Metabolism

Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Protein Glycosylation

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

Carbohydrate Catabolism

Carbohydrate Catabolism

Carbohydrate catabolism is a fundamental process in cellular metabolism that enables energy extraction from glucose through two primary pathways: cellular respiration and fermentation. Both pathways begin with glycolysis, which operates independently of oxygen availability.Glycolysis: A Shared Starting PointGlycolysis is an oxygen-independent process that breaks down glucose into two molecules of pyruvic acid. During this process, a net gain of two ATP molecules and two NADH molecules is...

Carbohydrate Digestion

Carbohydrate Digestion

Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. Carbohydrate digestion starts in the mouth during mastication, or chewing. The masticated carbohydrates remain intact in the stomach. Digestion resumes in the duodenum of the small intestine, where pancreatic alpha-amylase and brush border enzymes of the microvilli convert complex carbohydrates to monosaccharides. Finally, the monosaccharides...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026

Same author

Mapping galectin-3 ligands in human tear fluid establishes spliceoform-dependent lacritin binding.

Communications biology·2026

Same author

Metabolic and lipidomic stability in EDTA plasma from hospitalized patients: impact of refrigerated storage time and additional freeze-thaw cycle.

Metabolomics : Official journal of the Metabolomic Society·2026

Same author

Mass spectrometric profiling reveals alterations in N-Glycans and O-Glycans in Tay-Sachs disease under Autophagy-Induced conditions.

Glycoconjugate journal·2025

Same author

Mapping galectin-3 ligands in tear fluid establishes spliceoform-dependent lacritin binding.

bioRxiv : the preprint server for biology·2025

Same author

Sialoglycans modulate Siglec-5-TLR4 interactions in osteoarthritis.

iScience·2025

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

UniCarb-DB: a database resource for glycomic discovery.

Catherine A Hayes¹, Niclas G Karlsson, Weston B Struwe

¹Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Sweden.

Bioinformatics (Oxford, England)

|March 15, 2011

Summary

This summary is machine-generated.

This study introduces a new platform for analyzing oligosaccharide structures using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This resource addresses a gap in glycomic data analysis tools, aiding researchers in glycan structure profiling.

More Related Videos

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Published on: January 20, 2022

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Related Experiment Videos

Last Updated: Jun 3, 2026

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Published on: January 20, 2022

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

Area of Science:

Glycomics
Post-translational modifications
Mass spectrometry

Background:

Glycosylation is a critical protein modification involved in immune responses.
The field of glycomics lacks adequate tools for high-throughput data analysis compared to proteomics.
Existing tools for mass spectrometry (MS) and high-performance liquid chromatography (HPLC) are improving, but LC-MS/MS glycan profiling resources are scarce.

Purpose of the Study:

To present a novel platform for the analysis and presentation of oligosaccharide structures.
To provide a resource for glycan structure profiling using LC-MS/MS techniques.
To enhance existing glycomics databases and standards.

Main Methods:

Development of a platform for presenting oligosaccharide structures.
Characterization of fragment data using LC-MS/MS strategies.
Annotation of the database with high-quality datasets.

Main Results:

A new platform is available for presenting oligosaccharide structures and fragment data.
The platform supports LC-MS/MS strategies for glycan analysis.
The database is annotated with high-quality data.

Conclusions:

The developed platform addresses the need for better LC-MS/MS data analysis tools in glycomics.
This resource will aid in the structural profiling of glycans.
The platform aims to extend and reinforce standards in glycomics databases.