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Related Concept Videos

Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

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Global and Current Research Trends of Single-Cell Sequencing in Cancer: A Bibliometric and Visualization Study
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Tumour evolution inferred by single-cell sequencing.

Nicholas Navin1, Jude Kendall, Jennifer Troge

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.

Nature
|March 15, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces single-nucleus sequencing to accurately analyze genomic copy number variation in individual cells. This method reveals distinct clonal subpopulations and punctuated tumor evolution, offering new insights into cancer progression.

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Area of Science:

  • Genomics
  • Cancer Biology
  • Evolutionary Biology

Background:

  • Genomic analysis is crucial for understanding disease, but current methods struggle with mixed cell populations, especially in heterogeneous tumors.
  • Tumor genetic heterogeneity obscures evolutionary history, limiting insights into cancer progression.

Purpose of the Study:

  • To develop and apply a single-nucleus sequencing method for accurate genomic copy number quantification within individual cells.
  • To investigate tumor population structure and evolutionary dynamics in human breast cancer.

Main Methods:

  • Utilized flow-sorted nuclei, whole genome amplification, and next-generation sequencing.
  • Applied single-nucleus sequencing to analyze copy number variation at the single-cell level.
  • Examined two human breast cancer cases, analyzing 100 single cells from each.

Main Results:

  • Accurately quantified genomic copy number in individual nuclei, resolving mixed cell populations.
  • Identified three distinct clonal subpopulations in a polygenomic breast tumor, suggesting sequential expansions.
  • Revealed a single clonal expansion seeded metastasis in a monogenomic tumor and identified abundant, non-metastatic pseudodiploid cells.

Conclusions:

  • Single-nucleus sequencing is a powerful tool for dissecting tumor heterogeneity and evolution.
  • Tumor growth occurs through punctuated clonal expansions rather than gradual progression.
  • Identified distinct clonal architectures and a novel subpopulation of genetically diverse cells in breast tumors.