Related Experiment Video
Updated: Jun 3, 2026
Fluorescence-Based Detection of FEN1 Nuclease Activity and Screening of Small-Molecule Inhibitors
Published on: June 27, 2025
Ultrasensitive endonuclease activity and inhibition detection using gold nanoparticle-enhanced fluorescence
Yong Huang1, Shulin Zhao, Zhen-Feng Chen
1Ministry of Education Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China.
Abstract:
We have developed a highly sensitive and selective fluorescence polarization assay method based on the specificity of the DNA cleavage reaction with the enhancement of gold nanoparticles (AuNPs) for assaying endonuclease activity and inhibition. This assay can detect EcoRI endonuclease down to 5.0×10(-4) U mL(-1) with a detection range from 5.0×10(-4) to 10 U mL(-1).
