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Updated: Jun 3, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Fe/Mn superoxide dismutase-encoding gene in Paracoccus denitrificans is induced by azide and expressed independently
P Bouchal1, T Vyhlídalová, I Struhárová
1Department of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500, Brno, Czech Republic.
Abstract:
Paracoccus denitrificans cells undergo changes in protein composition upon exposure to azide, a known activator of the fumarate-nitrate reduction (FNR)-type transcription factor NarR. One of the most prominent protein species inducible by azide is a Fe/Mn-family superoxide dismutase (SOD). Azide induces SOD at protein, mRNA transcript, and enzyme activity levels in the aerobically growing cells. Since SOD expression remains unaffected in the fnrP-, nnr-, and narR-mutant strains, we postulate a mechanism independent of the known FNR-type regulators but involving a redox signal arising from the respiratory chain.
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