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BSA adsorption on titanium: ToF-SIMS investigation of the surface coverage as a function of protein concentration and

M Wilhelmi¹, C Müller, C Ziegler

¹Institute for Surface and Thin Film Analysis, Kaiserslautern, Germany. wilhelmi@ifos.uni-kl.de

Analytical and Bioanalytical Chemistry

|March 15, 2011

Abstract:

The adsorption properties of bovine serum albumin (BSA) on pure titanium (99.99%+) were studied by time-of-flight secondary ion mass spectrometry (ToF-SIMS) and X-ray photoelectron spectroscopy (XPS). For this purpose, films consisting of BSA were prepared on cleaned titanium sheets in a phosphate buffered solution under different conditions and analysed by ToF-SIMS. The dependence of the surface coverage on the concentration of the protein solution could be determined from the secondary ion mass spectrometry signals. The results agree satisfactorily with the corresponding results obtained by XPS. The observed adsorption behaviour can be fitted by the well-known formula describing a Langmuir isotherm. The Langmuir constant for pH 7 was found to be K = 174 ± 99 lg(-)1.

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