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J B Hammond1, N J Kruger

  • 1Biochemistry Department, Rothamsted Experimental Station, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2011
PubMed

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Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.