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Purification of immunoglobulins using protein a-sepharose

N J Kruger1, J B Hammond

  • 1Biochemistry Department, Rothamsted Experimental Station, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 15, 2011
PubMed

Most immunochemical techniques used in molecular biology rely on a specific class of antibodies, immunoglobulin G (IgG). In mammals, IgG antibodies are produced during the secondary humoral response and contribute about 80% of the serum immunoglobulin. Factors affecting the experimental production of antibodies have been discussed in detail (1). In this chapter we describe a method for the rapid and efficient purification of IgG to near homogeneity from rabbit serum. This is achieved by affinity chromatography using protein A.

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