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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

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Related Experiment Video

Updated: Jun 3, 2026

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Genetic manipulation of Clostridium difficile.

Laurent Bouillaut¹, Shonna M McBride, Joseph A Sorg

¹Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, USA.

Current Protocols in Microbiology

|March 15, 2011

Summary

Genetic manipulation techniques for Clostridium difficile, a common cause of colitis, have advanced significantly. This research details methods for gene disruption, complementation, and plasmid integration in this bacterium.

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Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

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Last Updated: Jun 3, 2026

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Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

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Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Published on: September 14, 2013

Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Clostridium difficile is an anaerobic, spore-forming bacterium responsible for antibiotic-associated colitis.
Historically, genetic manipulation of C. difficile was challenging.
Recent advancements have enabled genetic modification of this organism.

Purpose of the Study:

To describe molecular techniques for genetic modification of Clostridium difficile.
To provide methods for gene disruption, complementation, and plasmid manipulation.
To outline cross-species conjugation techniques for C. difficile.

Main Methods:

Gene disruption and complementation strategies.
Plasmid introduction, integration, and maintenance.
Cross-species conjugation protocols.

Main Results:

Established and adapted multiple genetic manipulation methods for C. difficile.
Demonstrated feasibility of gene disruption and complementation.
Successfully implemented plasmid introduction and integration techniques.
Showcased utility of cross-species conjugations.

Conclusions:

Molecular techniques for C. difficile genetic modification are now available.
These methods facilitate further research into C. difficile pathogenesis and treatment.
Advancements in genetic manipulation are crucial for understanding and combating C. difficile infections.