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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

High-throughput lectin microarray-based analysis of live cell surface glycosylation.

Yu Li¹, Sheng-Ce Tao^2,3,4, Heng Zhu^2,3

¹Department of Pathology, Oncology & Medicine and Institute for Cell Engineering, Johns Hopkins University, Baltimore, Maryland.

Current Protocols in Protein Science

|March 15, 2011

Summary

Lectins, which bind to sugars, can now be used in a high-throughput microarray format to analyze cell surface glycans. This method offers a powerful alternative for detailed glycosylation profiling.

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Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Published on: December 17, 2012

Area of Science:

Biochemistry
Glycobiology
Biotechnology

Background:

Lectins are plant-derived proteins that bind to specific carbohydrate structures (glycans).
Traditional methods for analyzing cell surface glycans, such as flow cytometry, are not high-throughput.
Existing lectin-based techniques have limitations in discriminating sugar isomers and scalability.

Purpose of the Study:

To present a novel lectin-based microarray approach for high-throughput analysis of cell surface glycosylation.
To demonstrate the feasibility of profiling accessible cell surface glycans on living cells.

Main Methods:

Printing lectins with diverse glycan specificities onto a glass substrate to create a microarray.
Utilizing the lectin microarray to profile accessible cell-surface glycans on living cells in a high-throughput manner.

Main Results:

The lectin microarray approach enables the analysis of a broad range of cell surface glycans.
This method is demonstrated to be feasible for identifying binding-accessible glycosylation on living cells.
The microarray format allows for high-throughput profiling, overcoming limitations of traditional methods.

Conclusions:

Lectin microarrays provide a high-throughput and adaptable platform for cell surface glycan analysis.
This technique offers a significant advancement for detailed glycosylation profiling and discovery.