[Procedure for Trichomonas vaginalis cultivation]

The investigation was undertaken to design a nutrient medium that could reduce the duration of a culture test in the diagnosis of trichomoniasis and to comparatively evaluate its effectiveness. A procedure was proposed to cultivate Trichomonas vaginalis, which was based on the use of the nutrient medium containing casein hydrolysate, yeast autolysate, and serum, added by 0.6 ml of liquid Ferrum Lec (iron (III) hydroxide polyisomaltose), which reduced the time taken to identify Trichomonas vaginalis (on day 2). At the same time, the higher content of iron [III] hydroxide with polyisomaltose was shown to produce no positive effect.