Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High concentrations of low density lipoprotein decrease basement membrane-associated heparan sulfate proteoglycan in

B Olgemöller1, E D Schleicher, S Schwaabe

  • 1Institut für Klinische Chemie und Diabetesforschung, Städtisches Krankenhaus Schwabing, München, FRG.

FEBS Letters
|May 7, 1990
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Spinal muscular atrophy : Time for newborn screening?]

Der Nervenarzt·2017
Same author

IL-6 deficiency in mice neither impairs induction of metabolic genes in the liver nor affects blood glucose levels during fasting and moderately intense exercise.

Diabetologia·2010
Same author

Activation of the mitogen-activated protein kinase (MAPK) signalling pathway in the liver of mice is related to plasma glucose levels after acute exercise.

Diabetologia·2010
Same author

Dissection of biochemical borderline phenotypes in carriers and genetic variants of medium-chain acyl-CoA dehyrogenase deficiency: implications for newborn screening [corrected].

Clinical genetics·2009
Same author

Selective association of plasma non-esterified fatty acid species with circulating interleukin-8 concentrations in humans.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association·2008
Same author

Increased levels of HPV16 E6*I transcripts in high-grade cervical cytology and histology (CIN II+) detected by rapid real-time RT-PCR amplification.

Cytopathology : official journal of the British Society for Clinical Cytology·2007

Increasing low density lipoprotein (LDL) levels impact basement membrane synthesis in porcine aortic endothelial cells (PAEC). High LDL concentrations decrease heparan sulfate proteoglycan (HSPG) content, while fibronectin synthesis remains largely unaffected.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Cardiovascular Research

Background:

  • Endothelial cells form the basement membrane, crucial for vascular health.
  • Low-density lipoproteins (LDL) are implicated in cardiovascular disease, but their direct effects on endothelial basement membrane synthesis are not fully understood.

Purpose of the Study:

  • To investigate the impact of varying low-density lipoprotein (LDL) concentrations on the synthesis of basement membrane components in cultured porcine aortic endothelial cells (PAEC).

Main Methods:

  • Porcine aortic endothelial cells (PAEC) were cultured and exposed to different extracellular LDL concentrations.
  • Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin levels were quantified using enzyme immunoassays.
  • Cell proliferation and metabolic activity were monitored via lactate production.

Related Experiment Videos

Main Results:

  • Low extracellular LDL levels led to an increase in HSPG content within PAEC.
  • Conversely, high extracellular LDL levels resulted in a decrease in PAEC HSPG content.
  • Fibronectin synthesis showed only minor alterations, and cell proliferation and metabolic activity remained constant across LDL concentrations. Insulin and high glucose did not modify LDL's effect on basement membrane components.

Conclusions:

  • LDL concentration significantly influences the synthesis of heparan sulfate proteoglycan (HSPG) in endothelial cells.
  • High LDL levels may negatively impact basement membrane integrity by reducing HSPG content, a finding potentially relevant to cardiovascular pathology.
  • The observed effects are specific to LDL concentration and not modulated by insulin or high glucose levels in this experimental model.