Related Experiment Video
Updated: Jun 3, 2026
08:08
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
Published on: November 19, 2020
DNA-unstable decaploid mouse H1 (ES) cells established from DNA-stable pentaploid H1 (ES) cells polyploidized using
K Fujikawa-Yamamoto1, M Miyagoshi, X Luo
1Division of Cell Medicine, Research Institute of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa, Japan. fujikawa@kanazawa-med.ac.jp
Cell Proliferation
|March 16, 2011
Summary
Decaploid H1 (ES) cells (10H1 cells) were created and found to be DNA-unstable, unlike their pentaploid (5H1) predecessors. This suggests DNA stability in polyploid cells is complex and may depend on DNA configuration.
Area of Science:
- Cell Biology
- Genetics
- Developmental Biology
Background:
- Diploid H1 (ES) cells exhibit stable DNA content in culture.
- Tetraploid and octaploid H1 (ES) cells demonstrate DNA instability.
- Pentaploid H1 (ES) cells are recently established and appear DNA-stable.
Purpose of the Study:
- To investigate the determinants of DNA stability in polyploid H1 (ES) cells.
- To establish and characterize decaploid H1 (ES) cells (10H1 cells) derived from pentaploid cells (5H1 cells).
- To assess the DNA stability of 10H1 cells during long-term culture.
Main Methods:
- Pentaploid H1 (ES) cells were polyploidized using demecolcine (DC).
- Decaploid H1 (ES) cells (10H1 cells) were obtained through single-cell cloning.
- Cell cycle phases, cell volume, morphology, alkaline phosphatase activity, and DNA content decay were analyzed.
Main Results:
- 10H1 cells possessed 180 chromosomes with G(1), S, and G(2)/M phases of 3, 7, and 6 hours, respectively.
- 10H1 cells were larger than 5H1 cells, exhibited flagstone-like morphology, and showed alkaline phosphatase activity.
- 10H1 cells displayed DNA content decay over 91 days and formed teratomas with differentiated cells, indicating pluripotency and DNA instability.
Conclusions:
- The transition from pentaploid to decaploid H1 (ES) cells retained cell cycle parameters and pluripotency.
- Morphology and DNA stability were significantly altered during the pentaploid-decaploid transition.
- A hypothesis suggests DNA instability arises from mirror-symmetric arrangement in reconstructed polyploid DNA.
