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Published on: October 4, 2024
Immobilization of thrombocytes on PCL nanofibres enhances chondrocyte proliferation in vitro
R Jakubova1, A Mickova, M Buzgo
1Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic. jakubova@biomed.cas.cz
Objectives:
The aim of this study was to develop functionalized nanofibres as a simple delivery system for growth factors (GFs) and make nanofibre cell-seeded scaffold implants a one-step intervention.
Materials And Methods:
We have functionalized polycaprolactone (PCL) nanofibres with thrombocytes adherent on them. Immobilized, these thrombocytes attached to nanofibre scaffolds were used as a nanoscale delivery system for native (autologous) proliferation and differentiation factors, in vitro. Pig chondrocytes were seeded on the thrombocyte-coated scaffolds and levels of proliferation and differentiation of these cells were compared with those seeded on non-coated scaffolds.
Results:
Immobilized thrombocytes on PCL nanofibres effectively enhanced chondrocyte proliferation due to time-dependent degradation of thrombocytes and release of their GFs.
Conclusions:
These simply functionalized scaffolds present new possibilities for nanofibre applications, as smart cell scaffolds equipped with a GF delivery tool.
