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B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
Luciana P Almeida1, Ana Pf Trombone, Julio Cc Lorenzi
1Department of Biochemistry and Immunology, Medical School of Ribeirão Preto, University of São Paulo, Brazil. arlete@fmrp.usp.br.
Background:
Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown.
Methods:
In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge.
Results:
In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice.
Conclusions:
These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model.
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