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Updated: Jan 20, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
Published on: March 21, 2022
Proteins bearing oxidation-induced carbonyl groups are not preferentially ubiquitinated
1Institute of Nutrition, Department of Nutritional Toxicology, Friedrich Schiller University Jena, Dornburger Straße 24, 07743 Jena, Germany.
Abstract:
A substantial part of soluble, oxidized proteins are degraded by the proteasome. However, it is still under debate whether these oxidized proteins are degraded by the 26S proteasome in an ubiquitin-dependent way or in an ubiquitin-independent way by the 20S proteasome. Therefore, we treated cells with H(2)O(2) and UV-A irradiation and detected protein carbonyls and ubiquitination by immunoblotting. Separation of ubiquitinated proteins from non-ubiquitinated reveals that most oxidized proteins are not ubiquitinated.
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