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Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
Cell-surface Signaling01:21

Cell-surface Signaling

Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
Types of Receptors: Cell Surface Receptors01:28

Types of Receptors: Cell Surface Receptors

Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

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Related Experiment Video

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Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Secretion-and-capture cell-surface display for selection of target-binding proteins.

J A Rakestraw1, D Aird, P M Aha

  • 1Celexion LLC, One Kendall Square, Suite 14302, Cambridge, MA 02139, USA. arakestraw@celexionbio.com

Protein Engineering, Design & Selection : PEDS
|March 16, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel cell-surface display system using Secretion and Capture Technology (SECANT™) to show complex proteins like antibodies on living yeast cells. This method successfully isolated specific antibody-displaying cells from a large mixed population.

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Published on: March 24, 2023

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Cell-surface display systems are crucial for protein engineering and discovery.
  • Existing methods may have limitations in displaying complex or full-length proteins.
  • The Secretion and Capture Technology (SECANT™) platform offers a new approach.

Purpose of the Study:

  • To introduce and validate the SECANT™ platform for cell-surface protein display.
  • To demonstrate the system's capability in displaying complex proteins, specifically full-length immunoglobulin G (IgG).
  • To showcase the efficiency of SECANT™ in isolating specific protein-displaying cells.

Main Methods:

  • Utilized in vivo biotinylation of target proteins.
  • Captured biotinylated proteins on avidin-coated parent cells.
  • Employed cell sorting techniques to isolate desired clones.
  • Demonstrated proof-of-concept using Saccharomyces cerevisiae.

Main Results:

  • Successfully displayed full-length Herceptin IgG on the surface of Saccharomyces cerevisiae cells.
  • Isolated specific Herceptin IgG-displaying yeast cells from a 10,000-fold excess of cells displaying a different antibody (lysozyme-binding).
  • Validated the SECANT™ platform's ability to handle complex protein display and facilitate cell selection.

Conclusions:

  • The SECANT™ platform is a viable and effective system for displaying complex proteins, including full-length IgG, on living cells.
  • This technology enables efficient isolation of specific antibody-displaying cells.
  • SECANT™ holds potential for applications in antibody discovery, protein engineering, and synthetic biology.