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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
Insertion sequence ISAba11 is involved in colistin resistance and loss of lipopolysaccharide in Acinetobacter
Jennifer H Moffatt1, Marina Harper, Ben Adler
1Department of Microbiology, Monash University, Victoria, Australia.
Abstract:
Infections caused by Acinetobacter baumannii are of increasing concern, largely due to the multidrug resistance of many strains. Here we show that insertion sequence ISAba11 movement can result in inactivation of the A. baumannii lipid A biosynthesis genes lpxA and lpxC, resulting in the complete loss of lipopolysaccharide production and high-level colistin resistance.
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