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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Molecular basis of macrolide, triamilide, and lincosamide resistance in Pasteurella multocida from bovine respiratory

Kristina Kadlec1, Geovana Brenner Michael, Michael T Sweeney

  • 1Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Höltystr. 10, 31535 Neustadt-Mariensee, Germany.

Antimicrobial Agents and Chemotherapy
|March 16, 2011
PubMed

Insights

Researchers identified three novel genes, erm(42), msr(E), and mph(E), responsible for macrolide-triamilide resistance in Pasteurella multocida. These genes confer resistance when transferred to susceptible bacteria.

Area of Science:

  • Veterinary Microbiology
  • Molecular Biology
  • Antimicrobial Resistance

Background:

  • The emergence of antibiotic resistance in bacterial pathogens poses a significant threat to animal and human health.
  • Pasteurella multocida is an important veterinary pathogen, and understanding its resistance mechanisms is crucial for effective treatment.
  • The specific mechanisms underlying macrolide-triamilide resistance in P. multocida remained largely uncharacterized.

Purpose of the Study:

  • To elucidate the genetic basis of macrolide-triamilide resistance in a multiresistant bovine isolate of Pasteurella multocida.
  • To identify and characterize novel resistance genes conferring macrolide and macrolide-triamilide resistance.

Main Methods:

  • Whole-genome sequencing of a multiresistant P. multocida isolate.
  • Identification and PCR amplification of putative resistance genes: erm(42), msr(E), and mph(E).
  • Cloning of identified genes into plasmid vectors and transformation into susceptible E. coli and P. multocida strains to assess conferred resistance.

Main Results:

  • Whole-genome sequencing revealed three novel resistance genes: erm(42) (rRNA methylase), msr(E) (macrolide transporter), and mph(E) (macrolide phosphotransferase).
  • The erm(42) gene conferred macrolide-lincosamide resistance.
  • The msr(E)-mph(E) gene combination conferred macrolide-triamilide resistance.

Conclusions:

  • Three novel genes, erm(42), msr(E), and mph(E), have been identified as key determinants of macrolide and macrolide-triamilide resistance in Pasteurella multocida.
  • These findings provide critical insights into the molecular mechanisms of resistance in this important veterinary pathogen.
  • The characterized resistance genes can be utilized for developing diagnostic tools and strategies to combat antimicrobial resistance in P. multocida infections.

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