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Updated: Jun 3, 2026
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
Published on: July 11, 2019
Two-photon in vivo imaging of cells
Daniel J Christensen1, Maiken Nedergaard
1The Institute of Optics, University of Rochester, Rochester, NY, USA. dan_christensen@urmc.rochester.edu
Abstract:
In vivo imaging of cells gives a glimpse into the world of biology in a natural setting unparalleled by any other venue. Two-photon imaging of fluorescently labeled cells has become the standard to obtain high-resolution, dynamic images of living specimens with great specificity. This review focuses on providing the reader with a short history of, and impetus behind, two-photon imaging, its working mechanics, and emerging technologies related to biological multiphoton imaging.
