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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

Topology optimized microbioreactors.

Daniel Schäpper¹, Rita Lencastre Fernandes, Anna Eliasson Lantz

¹Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, Building 229, 2800 Kgs. Lyngby, Denmark. daniel.schaepper@schappi.dk

Biotechnology and Bioengineering

|March 16, 2011

Summary

This summary is machine-generated.

Topology optimization significantly enhances brewer's yeast (Saccharomyces cerevisiae) microbioreactor productivity by optimizing cell distribution. This method boosts recombinant protein yield, especially in nutrient-limited or inhibition-prone environments.

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Area of Science:

Biotechnology
Chemical Engineering
Computational Science

Background:

Microbioreactors are essential for bioprocessing, but homogeneous cell distribution can limit productivity.
Saccharomyces cerevisiae is widely used for recombinant protein production.
By-product inhibition and nutrient scarcity can significantly reduce efficiency in bioreactors.

Purpose of the Study:

To integrate topology optimization with microbioreactor design.
To spatially redistribute immobilized yeast cells for maximal product flow.
To mitigate negative effects like by-product inhibition.

Main Methods:

Utilized topology optimization algorithms to redesign cell distribution within a rectangular microbioreactor.
Modeled Saccharomyces cerevisiae immobilized cell behavior and recombinant protein production.
Incorporated factors such as nutrient transport and by-product inhibition into the optimization model.

Main Results:

Achieved a theoretical productivity increase of at least fivefold compared to homogeneous cell distribution.
Demonstrated that topology optimization yields the greatest improvements in nutrient-scarce or inhibition-heavy conditions.
Identified optimal non-homogeneous cell distributions for enhanced product outflow.

Conclusions:

Topology optimization offers a powerful approach to enhance microbioreactor performance.
Spatially optimized cell distribution can overcome limitations of homogeneous systems.
This integrated methodology holds significant potential for improving bioprocess efficiency and recombinant protein yield.