[Determination of enoximone and its principle metabolite in serum and urine using high pressure liquid...

We developed a high performance liquid chromatography method for the monitoring of enoximone and its main metabolite in serum and urine. Samples handling involves a unique chemical extraction step by ethylacetate. Serum needs at first to be deproteinized by acetonitril. The chromatographic separation is realized on a reversed phase analytical column by gradient elution with acetonitrile. Quantification is by U.V. absorbance at 365 nm. We compared our new method with the method so far considered as reference one. When specificity, accuracy and linearity of both procedures are similar, we greatly enhanced the detection limit [(5 ng/ml for (E) and (SE)] and the practicability: ease of use, rapidity and lower cost.