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Updated: Jun 3, 2026

Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
Published on: October 1, 2016
Improvement of egg hatch of Ceratitis capitata (Diptera: Tephritidae) for enhanced output
Meriem M'Saad Guerfali1, Heithem Hamden, Selma Fadhl
1Unité de Production des mâles Sériles de la Cératite, Centre National des Sciences et Technologies Nucléires, Technopole Sidi Thabet, 2020, Tunis, Tunisie. msaad_tn@yahoo.fr
Abstract:
Embryonated eggs of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) genetic sexing strain (GSS), VIENNA 8 were treated with low concentrations of five disinfectants-formaldehyde, iodine, sodium hypochlorite, peracetic acid, and quaternary ammonium--for decontamination and egg hatch improvement. The newly laid eggs were successfully treated with formaldehyde at 100 ppm for 1 min with 74.2% hatching and with quaternary ammonium at 150 ppm for 1 and 2 min with 70.4 and 69% hatching, respectively. Increased formaldehyde concentration may have affected the embryos, because it resulted in a decrease in the hatching percentage. However, egg viability was not impaired and hatch was not affected by quaternary ammonium treatment compared with controls and eggs treated with other disinfectants. Quaternary ammonium shows promise for decontaminating eggs and improving egg hatch.

