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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Related Experiment Video

Updated: Jun 3, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Evolution of a tissue-specific splicing network.

J Matthew Taliaferro1, Nehemiah Alvarez, Richard E Green

  • 1Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.

Genes & Development
|March 17, 2011
PubMed
Summary
This summary is machine-generated.

A new splicing factor, LS2 (Large Subunit 2), evolved from dU2AF(50) in Drosophila. LS2 represses splicing and is specific to testes, contributing to tissue-specific transcript diversity.

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Related Experiment Videos

Last Updated: Jun 3, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

  • Molecular Biology
  • Evolutionary Biology
  • Genetics

Background:

  • Alternative splicing of precursor mRNA (pre-mRNA) enhances proteomic diversity in eukaryotes.
  • Metazoan splicing factors often belong to multigene families, but their functional divergence remains unclear.

Purpose of the Study:

  • To characterize the evolution and function of a novel Drosophila splicing factor, LS2.
  • To understand how related splicing factors acquire unique properties and tissue-specific roles.

Main Methods:

  • Gene duplication analysis to identify LS2.
  • RNA binding assays to determine RNA sequence specificity.
  • Splicing assays to assess LS2's regulatory function.
  • Expression pattern analysis to determine tissue specificity.

Main Results:

  • LS2 evolved from a duplication of the dU2AF(50) gene.
  • LS2 exhibits distinct RNA binding preferences compared to dU2AF(50).
  • LS2 functions as a splicing repressor and is preferentially expressed in Drosophila testes.
  • LS2 targets transcripts involved in testes-specific functions.

Conclusions:

  • LS2 represents a novel splicing factor that evolved unique regulatory functions.
  • The evolution of LS2 provides a model for how splicing factors gain tissue-specific roles.
  • LS2 contributes to transcriptomic diversity in a testes-specific manner through pre-mRNA splicing regulation.