Ionic concentration- and pH-dependent electrophoretic mobility as studied by single colloid electrophoresis

Optical tweezers are employed to measure separately the complex electrophoretic mobility of a single colloid and the complex electroosmotic response of the surrounding medium in a specially designed microfluidic cell. Using the very same colloid both quantities are determined in dependence on the concentration of the aqueous salt solution (10 (- 5)-10 (- 1) mol l (- 1)), the valence of the ions (K (+) , Ca(2 +) ) and the pH (2.5-8.5). A pronounced effect is observed for all these examined parameters. The dependence on ion concentration agrees qualitatively-for the monovalent case-with the predictions of the standard electrokinetic model.